CSIR-Central Leather Research Institute
Dr.N Ayyadurai
Senior Scientist
Academic Qualifications
    Ph.D, Pondicherry University
Contact Details
Dr.N Ayyadurai
Senior Scientist

Biochemistry & Biotechnology
CSIR-Central Leather Research Institute
Adyar, Chennai – 600 020
e-mail: ayyadurai@clri.res.in,
Phone:91 44 24437136


Ph.D. in Biotechnology : 2001-2006, Pondicherry University (Central University), Pondicherry, India

Master in Microbiology : 1998-2000, Madurai Kamaraj University, Madurai, Tamil Nadu, India

Area of interests
  • Proteomic and genomics approach in pre and post tanning process;,
  • expanding and engineering the genetic code tools in leather processing and enzyme engineering

Our mission is to expand the number of the amino acids (AA) for ribosomal protein synthesis through recent chemical biology approach. This requires the reprogramming of the protein translation machinery by changing the coding capacities of the standard genetic code. Our research at the line of chemistry and biology is aimed towards the generation of novel generic classes of biocatalyst and congener proteins by using synthetic biology, Xenobiology and target-engineered metabolic circuits. These mainly synthetic amino acid building blocks. 

Our Research focus

  • Genetic code engineering/expansion for ribosomal translation, synthetic biology of bio-orthogonal chemistries for protein modification and bioconjugation.
  • Exploring the genetic incorporation of NCAA to study protein stability/folding, multivalent binding, conformational dynamics & optical properties.
  • Bio-expression of genetically encodable complex biomaterials for tissue engineering.
  • Preparing new biocatalyst, through coupling synthetic biology and metabolic engineering for artificial biological diversity and technologies derived therefrom.
Universal Life Codon

Our Approach for Protein Modification

Our Model Protein

Antibody-Drug Conjugates

Professional Experience

August 2013 Senior Scientist, Department of Biotechnology. Central Leather Research Institute, Adyar, Chennai
2012-2013 Assistant Professor, Department of Biochemistry, King Saud University, Riyadh, Saudi Arabia
2009- 2012 Assistant Foreign Professor, School of Biotechnology, Yeungnam University, South Korea
2007-2009 Postdoctoral Researcher, Division of Bimolecular Engineering, Pusan National University, S.Korea
2006-2007 Invited Scientist, Biocentre division, Korea Polar Research Institute (KOPRI), S, Korea
2001-2006 Ph.D. in Biotechnology, Pondicherry Central University, India

Membership in Society

  • Member of the protein Society, United States of America
  • Editorial Board Member in Research Journal of Biotechnology [SCIE journal]
  • Member of Korean Biotechnology and Bioengineering society [KSBB]
  • Member of Biotech Research Society of India [BRSI]
  • Member of Association of Microbiologist in India[AMI]
  • Member of Korean Chemical Society in Republic of Korea


  1. Asuma Janeena J, Ilamaran M, George A, George SA, Sriram Raghavan S, Surya Lakshmi P, Aarthy M, Kamini NR and Gunasekaran K, Ayyadurai N.
    Biomimetic strategies to design metallic proteins for detoxification of hazardous heavy metal.
    Journal of Hazardous Materials. 358; 92–100. [Impact factor 6.4].

  2. M. Aarthy, P. Saravanan, N. Ayyadurai, M. K. Gowthaman, N. R.Kamini.
    A green process for starch oleate synthesis by Cryptococcus sp. MTCC 5455 lipase and its potential as an emulsifying agent.
    Starch (In Press). [Impact factor 1.8].
  3. Reddy S, George A, N. Ayyadurai, S Ganesh.
    Biocytin based pH-stimuli responsive supramolecular multivariant hydrogelator. Soft Matter (In Revision)
  4. A. Raman, George A, N. Ayyadurai and S. Easwaramoorthi.
    Photoswitchable Azobenzene-Rhodamine Tweezers for Biosensing of Al3+ Ions.
    New Journal of Chemistry. [Impact factor 3.8].

  5. P. Puhazhselvana, R. Aparna, N. Ayyadurai, N. R. Kamini.
    Growth associated degradation of aliphatic-aromatic copolyesters by Cryptococcus sp. MTCC 5455.
    Polymer Degradation and Stability. 152; 20-28. [Impact factor 1.8].

  6. 2017

  7. Reddy SMM, Dorishetty P, Augustine G, Deshpande AP, Ayyadurai N, Shanmugam G.
    A Low-Molecular-Weight Gelator Composed of Pyrene and Fluorene Moieties for Effective Charge Transfer in Supramolecular Ambidextrous Gel.
    Langmuir. 28:13504-13514. [Impact factor 3.8].

  8. Sophea Pheng, N. Ayyadurai, A-Young Park, Song-Gun Kim.
    Psychrosphaera aquimarina sp. nov., a marine bacterium isolated from seawater collected from Asan Bay, Republic of Korea.
    International Journal of Systematic and Evolutionary Microbiology 67:4820-4824. [Impact factor 3.2].
  9. George A, Krishna Priya G, Ilamaran M, Kamini NR, Ganesh Shanmugam, Shanmugam Easwaramoorthi and N. Ayyadurai.
    Accelerated strain-promoted and oxidation-controlled cyclooctyne-quinone cycloaddition for cell labeling.
    Chemistryselect 2: 7117–9 (IF Waiting).

  10. P. Puhazhselvana, R. Aparna, N. Ayyadurai, M. K. Gowthaman, P. Saravanan, N. R. Kamini.
    Enzyme based cleaner process for enhanced recovery of lipids from tannery fleshing waste.
    Journal of Cleaner Production 144, 187–191. [Impact factor 5.7].
  11. K. Deepankumar, A. George, G Krishna Priya, M Ilamaran, N.R.Kamini, T.S.Senthil, S. Easwaramoorthi and N. Ayyadurai.
    Next Generation Designed Protein as a Photosensitizer for Biophotovoltaics Prepared by Expanding the Genetic Code.
    ACS Sustainable Chemical Engineering 5, 72–77. [Impact factor 5.9].

  12. 2016

  13. Balijapalli U, Udayadasan S, George A, S Easwaramoorthi, N Ayyadurai, K I Sathiyanarayanana.
    Photophysical studies of donor, acceptor substituted tetrahydrodibenzo[a,i]phenanthridines.
    Dyes and Pigments. 134: 409–418. [Impact factor 4.0].
  14. Krishna Priya G, Mohammed Abu Javid M, George A, Aarthy M, Durai Anbarsan S, Kamini NR, Gowthaman MK, Aravindhan R, Ganesh S, Chandrasekar R and Ayyadurai N.
    Next generation greener leather dyeing process through recombinant green fluorescent protein.
    Journal of Cleaner Production. 126: 698-706. [Impact factor 5.7].

  15. M. Aarthy, P. Saravanan, N. Ayyadurai, M. K. Gowthaman, N. R. Kamini.
    A two step process for production of omega 3-polyunsaturated fatty acid concentrates from sardine oil using Cryptococcus sp. MTCC 5455 lipase.
    Journal of Molecular Catalysis B:Enzymatic : 125: 25–33.[Impact factor 2.8]

  16. 2015

  17. S. Pavithra, R. Ramesh, M. Aarthy, N. Ayyadurai, M. K. Gowthaman, N. R. Kamini.
    Starchy substrates for production and characterization of Bacillus subtilis amylase and its efficacy in detergent and bread making formulations.
    Starch 66976–984.[Impact factor 1.4]

  18. 2014

  19. Yang B, Ayyadurai N, Yun H, Choi Y. S, Hwang B. H, Huang J, Lu Q, Zeng H, Cha HJ.
    In vivo: residue-specific Dopa-incorporated engineered mussel bioglue with enhanced adhesion and water resistance.
    Angew Chemie Int. Ed. (In Press). [Impact factor 13.7]

  20. Deepankumar K, Prabhu NS,  Ayyadurai N, Song E, Lee N, Kim SK, Kim BG, Soundrarajan N, Lee S, Cha HJ, Budisa N, Yun H.
    Enhancing thermostability and organic solvent tolerance of ω-transaminase through global incorporation of fluorotyrosine.
    Advanced Synthesis and Catalysis. 356: 993-998. [Impact factor 5.5].

  21. 2013

  22. Deepankumar K, SP Nadarajan, N. Ayyadurai, Yun H.
    Enhancing the biophysical properties of mRFP1 through incorporation of fluoroproline.
     Biochem Biophys Res Commun440:509-414. [Impact factor 2.4]

  23. 2012

  24. N. SaravananPrabhu, N. Ayyadurai (Equally contributed), K. Deepankumar, T. Chung, D. Joon Lim, H. Yun.
    Reassignment of sense codons: Designing and docking of proline analogs for Escherichia coli prolyl-tRNA synthetase to expand the genetic code.
    J. Mol. Catal. B: Enzym 78:57–64. [Impact factor 2.8].
  25. N. Ayyadurai, K. Deepankumar, N. SaravananPrabhu, N. Budisa, and H. Yun.
    Evaluation and biosynthetic incorporation of chlorotyrosine into recombinant proteins.
    Biotechnol. Bioprocess Eng.17: 679-686. [Impact factor 1.2].
  26. N. Soundrarajan, S. Edwardraja, Sun-Gu Lee, H. Yun, N. Ayyadurai.
    Enhancing the productivity of soluble green fluorescent protein through methionine-residue specific consensus approach.
    Afr. J. Biotech. 11:1059-1064.[Impact factor 0.6]
  27. Chenniappan K, N.Ayyadurai (Equal contributed). Synergistic activity of Cyt1A from Bacillus thuringiensis subsp. israelensis with Bacillus sphaericus B101 H5a5b against Bacillus sphaericus B101 H5a5b-resistant strains of Anopheles stephensi Liston (Diptera: Culicidae). Parasitology Research.110: 381-388. [Impact factor 2.8].
  28. N. RameshKumar, N. Ayyadurai(Equally contributed), N. Kayalvizhi1, P. Gunasekaran.
    Genotypic and phenotypic diversity of PGPR fluorescent pseudomonads isolated from the rhizosphere of sugarcane (Saccharumofficinarum L.).
    J. Microbiol. Biotech.22:17-28 [Impact factor 1.7]

  29. 2011

  30. N. Ayyadurai, N. SaravananPrabhu, K. Deepankumar, Heon-Ho Jeong, Chang-Soo Lee, S. G. Lee, and H. Yun.
    Genetic incorporation of metal binding site in green fluorescent protein for development of selective, sensitive and reversible protein based biosensor.
    AngewChemie Int. Ed.50:6534-6537[Impact factor 13.7]
  31. N. Ayyadurai, K. Deepankumar, N. SaravananPrabhu, S. G. Lee, and Hyungdon Yun.
    A facile and an efficient method for the incorporation of multiple unnatural amino acids into a single protein.
    ChemComm. 47:3430-3432. [Impact factor 6.3].
  32. N. Ayyadurai, N. SaravananPrabhu, K. Deepankumar, Jang YJ, Chitrapriya N, Song E, Lee N, Kim SK, Kim BG, Soundrarajan N, Lee S, Cha HJ, Budisa N and Yun H.
    Bioconjugation of L-3,4-Dihydroxyphenylalanine Containing Protein with a Polysaccharide.
    Biconjugate Chem. 22; 551-555. [Impact factor 4.5].
  33. N. Ayyadurai, N. SaravananPrabhu, K. Deepankumar, S. G. Lee, and Hyungdon Yun.
    Biosynthetic substitution of tyrosine in green fluorescent protein with its surrogate fluorotyrosine in Escherichia coli.
    Biotechology Letters. 33:2201-2207. [Impact factor 1.8].

  34. 2010

  35. S. Nagasundarapandian, L. Merkel, N. Budisa, R. Govindan, N. Ayyadurai, S. Sriram, H. Yun, S. G. Lee.
    Engineering protein sequence composition for folding robustness renders efficient non-canonical amino acid incorporations.
    Chembiochem. 11:2521-2524. [Impact factor 3.7].
  36. J. Pathma, N. Ayyadurai, N. Sakthivel.
    Assessment of genetic and functional relationship of antagonistic fluorescent pseudomonads of rice rhizosphere by repetitive sequence, protein coding sequence and functional gene analyses.
    J. Microbiol. 48:715-727.[Impact factor 1.2].
  37. N. Ayyadurai, H.Yun, S. G. Lee.
    Role of Chaperon Co-Expression System in the Production of Recombinant Unnatural Proteins in Escherichia Coli.
    Res. J. Biotech. 5:49-53.[Impact factor 0.28].
  38. N. Ayyadurai,ValarmathyNatarajan and H. Yun.
    Effect of IndigoferaTinctoria against carbon tetrachloride induced hepatic damage in albino rats.
    Res. J. Biotech. 5: 5-8.[Impact factor 0.28].

  39. 2009

  40. N. Ayyadurai, H. Yun, and S. G. Lee.
    Application of Expanding the Genetic Code.
    Res. J. Biotech. 4: 76-77.[Impact factor 0.28].
  41. N. Ayyadurai, So Yeon Kim, and S. G. Lee.
    Biological Synthesis of Alkyne-terminated Telechelic Recombinant Protein.
    Macromolecular Res. 17: 424-429.[Impact factor 1.6].
  42. N. Ayyadurai,N. Ramesh Kumar, N. E. Selvakumar, R. Sundar and S. G. Lee.
    Importance of expression system in the production of unnatural recombinant proteins in Escherichia coli.
    Biotech. Bioproc. Eng. 14: 257-265.[Impact factor 1.0].

  43. 2008

  44. Ravindra Naik, Nirakar Sahoo, Devrishi Goswami, N. Ayyadurai,N. Sakthivel.
    Assessment of genetic and functional diversity among fluorescent pseudomonads isolated from the rhizosphere of banana.
    Microbial Ecol. 56: 492-504.[Impact factor 2.8].
  45. So-yeon Kim, N. Ayyadurai,Mi-aeHeo, Sunghoon Park, Yong joojeong and S. G. Lee.
    Improve the thermo tolerance of Escherichia coli by over expressing GroELS.
    J. Microbiol. Biotech 19: 72-77.[Impact factor 1.2].

  46. 2007

  47. N. Ayyadurai, P. RavindraNaik and N. Sakthivel.
    Functional characterization of antagonistic fluorescent pseudomonads associated with rhizospheric soil of rice (Oryza sativa L).
    J. Microbiol. Biotech17:919-927.[Impact factor 1.0].

  48. 2006

  49. N. Ayyadurai, P. Ravindra Naik, M. Sreehari Rao, R. Sunish Kumar, Subodh Kumar Samrat, M. Manohar, N. Sakthivel.
    Isolation and characterization of a novel banana rhizosphere soil bacterium as fungal antagonist and microbial adjuvant in micropropagation of banana.
    J. Appl.Microbiol. 100:926-937.[Impact factor 2.3].
  50. VirBahadur Singh, Anil Kumar, S. Isaac Kirubakaran, N. Ayyadurai, R. Sunishkumar and N. Sakthivel.
    Comparison of exopolysaccharides produced by Xanthomonasoryzaepv. oryzae strains, BXO1 and BXO8 that show varying degree of virulence in rice (Oryza sativa L.).
    J. Phytopathol. 154: 410-413.[Impact factor 0.9].

  51. 2005

  52. N. Ayyadurai, S.IssacKirubakaran, S.Srisha, N. Sakthivel.
    Biological and Molecular variability of Sarocladiumoryzae, the sheath rotpathogen of rice (Oryza sativa L.).
    Current Microbiol. 50:1-6.[Impact factor 1.5].
  53. R. Sunish Kumar, N. Ayyadurai, P.Pandiaraja, A. V. Reddy, Y. Venkateswarlu,Om Prakash,N. Sakthivel.
    Characterization of antifungal metabolite produced by a new strain Pseudomonas aeruginosaPUPa3 that exhibits broad-spectrum antifungal activity and biofertilizing traits.
    J. Appl. Microbiol. 98: 145-154. [Impact factor 2.3].

  54. 2004

  55. S. Datta, R. Sunish Kumar, N. Ayyadurai, Sujeet Kumar, N. Sakthivel.
    Polysaccharides of Pseudomonas pathovar strains that infect pea, tomato and soya bean.
    Current Microbiol.49:35–41. [Impact factor 1.5].
  • 3,4-dihydroxy-L-phenylalanine incorporation for protein based biosensor generation; April, 2011, Patent Number: DP110321KR, South Korea
  • Protein polysaccharide conjugation by invivo incorporation of 3,4-dihydroxy-L-phenylalanine into proteins. April, 2011, Patent Number: DP110322KR, South Korea.

Awards & Honours Received

  • Finn Wold Travel award offered by The Protein Society to attend 25th anniversary symposium of the protein society is held in Boston, United States of America (USA)(2011).
  • International Speaker award to attendInternational conference on Greenomics arranged by School of Life Sciences, Hindustan University, Chennai, India. (2010)
  • Welcome trust international travel award to attend Open door workshop in Bangkok. (2009.)
  • International Postdoctoral Fellowship award from Korean science and Engineering, (KOSEF) S. Korea (2008).
  • University Postdoctoral Fellow award from Pusan National University, S. Korea (2007).
  • Senior Research Fellowship from Council of Scientific and Industrial Research (CSIR), Government of India (2005 - 2006).
  • Research Fellowship from University Grant Commission, Government of India (2001-2004)
  • Mayhco Research Fellowship Award for Attending International Conference, India (2005).


Ph.D Students


George Augustine, CSIR-SRF
Title: Protein Modification through expanding the genetic code and synthetic route for ribosomal synthesis of fluorescent protein variants

Ilamaran Meganathan, CSIR-SRF
Title: Recombinant collagen for tissue engineering

Asuma Janeena, CSIR-JRF
Title: Antibody-drug conjugates and expanding the genetic code for protein based cancer therapy

Suryalakshmi Pandurangan, DST Inspire-JRF
Title: Enzyme engineering and protein nanofibers for biomedical applications

Sisila Valappil, CSIR-JRF
Title: Xenobiology and biosynthesis of Synthetic Proteins

Karthik Shankar, MS by Research
Title: DNA barcoding and synthetic biology of enzyme for leather applications