CSIR-Central Leather Research Institute
Dr.V.G Vaidyanathan
Senior Scientist
Academic Qualifications
    PhD
Contact Details
Dr.V.G Vaidyanathan
Senior Scientist

Advanced Materials Laboratory
CSIR-Central Leather Research Institute
Adyar, Chennai – 600 020
e-mail: vaidyanathan@clri.res.in,
Phone:+91 44 24437142


Qualification
 

Ph.D., University of Madras, India (2005) Dissertation entitled “Role of Ligand Structure in the Interaction of Chromium(III) Complexes with DNA”, Advisor: Dr. Balachandran Unni Nair, Central Leather Research Institute, Adyar, Chennai

M.Sc (Chemistry) University of Madras (2000)

B.Sc (Chemistry) University of Madras (1998)

Area of interests
 
  • Bioinorganic Chemistry
  • Biophysics.
  • Chemical Carcinogenesis
  Associate Professor, Academy of Scientific and Innovative Research (AcSIR)

Subjects Covered: Basic Chemistry, Biotechniques and Instrumentation, Research Methodology, Mass Spectrometry

  Faculty Member (Hon), Anna University

Subjects Covered: Instrument methods of analysis

Professional Experience
 

Associate Professor, Academy of Scientific and Innovative Research (AcSIR)
Subjects Covered: Stereochemistry of Organic Compounds, Drug Discovery

 

Faculty Member (Hon), Anna University
Subjects Covered: Instrument methods of analysis

  Ramanujan Fellow, CSIR-Central Leather Research Institute, “Investigation of Chromium(III)-DNA adducts in DNA Replication/Repair Machinery: Role of Ligand Structure in Chromium (III) Toxicity” -
April 2012 to current
  Visiting Professor, Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, USA -
Sep 13 to Nov 13
 

Postdoctoral Research Fellow, Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Project entitled: “Understanding the Sequence context of aminofluorene-adducted DNA at the Replication Fork By Biochemical and Biophysical Approaches” - Sep 08 to Apr 12

  Postdoctoral Research Associate, Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Worked on the project entitled: “Development of Chemical Strategies to Probe Carcinogenesis Mechanisms” -
Sep 05 to Aug 08
Research Interest
 
  • Area of research interests has been divided into two parts: (i) Understanding the role of Cr(III) adducts in replication and repair machinery at molecular level and (ii) Development of transition metal complexes as probes for detecting different DNA adducts and its conformations.

(i) Understanding the role of Cr(III) adducts in replication and repair machinery at molecular level

Chromium has been extensively used in dyes, chrome plating and leather industries. Chromium in +3 oxidation state is susceptible to form different DNA adducts including DNA interstrand crosslinks (ICLs), DNA-protein crosslinks (DPCs) as well as with DNA phosphates.  Though various reports have shown that DPCs exhibit different mutagenicity, the detailed mechanistic pathway is not yet ascertain. Our hypothesis is that the nature of adducts influence DNA replication fork progression and repair pathway in a sequence dependent manner. We use both biophysical as well as biochemical techniques to understand the mechanistic pathway. 

(ii) Development of transition metal complexes as probes for detecting different DNA adducts.

Transition metal complexes have been extensively studied as probes for DNA structures, photodynamic therapy and anticancer agents. However, only few studies have been reported on transition metal complexes as probes for the damaged site viz., abasic sites. The classical hypothesis of the DNA-adduct chemistry is that each DNA adduct adopts different conformations and each adduct conformation leads to multiple different mutations. The majority of toxicology research has been focused on determining the DNA adduct conformation using NMR as a key tool. The disadvantage of this method is (i) it requires large quantity of samples, (ii) time consuming and (iii) complexity in structural analysis. To overcome these drawbacks, we are interested in developing transition metal complexes as a tool to identify the conformations of the DNA adduct in a sequence dependent context.

 

Professional Affiliations
  Member of American Chemical Society (ACS) / Division of Chemical Toxicology
 

Life Member of Chemical Research Society of India (CRSI)

 

Lifetime Member of Indian Society of Nanomedicine (ISNM)

Research Grant
 
  • Investigation of Chromium(III)-DNA adducts in DNA Replication/Repair Machinery: Role of Ligand Structure in Chromium (III) Toxicity
    Funded by: Department of Science and Technology
    Amount: Rs. 8,285,000.00 (Rupees Eighty Two Lakhs Eighty Five Thousand)
    Duration: April 2012-Mar 2017
Book Chapter
 
  • Recent Advances in Gene Expression and Regulation
    Title: Chromium (III): Bio-significant or Bio-toxic?
    Authored by: Yamini Asthana, V.G. Vaidyanathan and B.U. Nair
    Published by: Nova Publishers (2013)

Patent Filed
 
  • TITLE: Platinum(II) complex as Cell Staining Agent
    INVENTORS:       V.G. Vaidyanathan*
                                 V.M. Manikandamathavan
                                  M.S.  Kiran
                                  B.U. Nair

Complete List of Publications

  1. J. Dhanya, B. Babu and V.G. Vaidyanathan* (2017) Effect of Chromium(III) gallate on the stabilization of collagen Int. J. Biol. Macromol., In press
  2. T. Nandhini, V.G. Vaidyanathan* and B.U. Nair (2016) Effect of Conformation of the arylamine-DNA adduct on the sensitivity of [Ru(phen)2(dppz)]2+ complexInorg. Chem. Commun., 73, 64-
  3. B. Babu, T. Nandhini, V.G. Vaidyanathan* and B.U. Nair (2016) Studies on interaction of Cr(III) polypyridyl complexes with DNA Inorg. Chem. Commun., 73, 124-128
  4. T. Nandhini, K.R. Anju, V. M. Manikandamathavan, V.G. Vaidyanathan* and B.U. Nair (2015) Interaction of Ru(II) polypyridyl complexes with DNA mismatches and abasic sites
    Dalton Trans.,44, 9044-9051
  5. V.M. Manikandamathavan, N. Duraipandy, M.S. Kiran, V.G. Vaidyanathan* and B.U. Nair (2015) A new Pt(II) complex for bioimaging applications
    RSC Adv.,5, 24877–24885
  6. L. Xu^, V.G. Vaidyanathan^ and B.P. Cho (2014) Real-time surface plasmon resonance study of biomolecular interactions between polymerase and bulky mutagenic lesions
    Chem. Res. Toxicol. 27, 1796-1807 (^ Authors contributed equally)
  7. V.Jain, V.G. Vaidyanathan, S. Patnaik, S. Gopal and B.P. Cho (2014) Conformational insights into the lesion and sequence effects for arylamine-induced translesion DNA synthesis: 19F NMR, Surface plasmon resonance and primer kinetic studies
    Biochemistry, 53, 4059-4071
  8. S. Rajalakshmi, M.S. Kiran, V.G. Vaidyanathan, E. R. Azhagiya Singam, V. Subramanian and B.U.Nair (2013) Investigation of nuclease, protelytic and anti-proliferative effects of copper(II) complexes of thiophenylmethanamine derivatives
    Eur. J. Med. Chem., 70, 280-293
  9. V.G. Vaidyanathan, F. Liang, W. Beard, D.D. Shock, S.H. Wilson and B.P. Cho (2013) Insights into the Conformation of Aminofluorene-dG Adduct in a DNA Polymerase Active Site
    J Biol. Chem. 288, 23573-23585
  10. J.-G. Dinorah, L. Li, V.G. Vaidyanathan, R. King, B. P. Cho, D. R. Worthen, C. O. Chichester III and N. P. Seeram (2013) Inhibitory Effects of the Pomegranate Polyphenol, Punicalagin, on Type-II Collagen Degradation In Vitro and Inflammation In Vivo
    Chemico-Biol. Interact., 205, 90-99
  11. V.G. Vaidyanathan*, L. Xu and B. P. Cho* (2013) Binding kinetics of DNA-protein interaction using surface plasmon resonance
    Nature Protocol Exchange doi:10.1038/protex.2013.054.
  12. V.G. Vaidyanathan, Yamini Asthana and B. U. Nair (2013) Importance of Ligand Structure in DNA/Protein Binding, Mutagenicity, Excision Repair and Nutritional Aspects of Chromium (III) Complexes
    Dalton Trans., 42, 2337-2346.
  13. V.G. Vaidyanathan, L. Xu and B. P. Cho (2012) Binary and ternary binding affinities between exonuclease-deficient Klenow fragment (Kf-exo−) and various arylamine DNA lesions characterized by surface plasmon resonance
    Chem. Res. Toxicol., 1568-1570.
  14. S.Sangeetha, G.Sathyaraj, M.Duraisamy, V.G.Vaidyanathan and B.U.Nair (2012) Structurally modified 1,10-phenanthroline based fluorophores for specific sensing of Ni2+ and Cu2+ ions
    Dalton Trans., 41, 5769-5773. (Top 10 Most Accessed Article in April 2012)
  15. V.G. Vaidyanathan and B.P.Cho (2012) Sequence effects on translesion synthesis of an aminofluorene-DNA adduct: conformational, thermodynamic, and primer extension kinetic studies
    Biochemistry, 51, 1983-1995.
  16. H.Dahlmann, V.G.Vaidyanathan and S.J.Sturla (2009) Investigating the Biochemical Impact of DNA Damage with Structure-Based Probes: Abasic Sites, Photodimers, Alkylation Adducts, and Oxidative Lesions
    Biochemistry, 48, 9347-9359 (Top 20 Most Read Article Nov 2009).
  17. V.G.Vaidyanathan, P.W.Villalta and S.J.Sturla (2007) Identification of Covalent DNA Adducts In Vitro From a Quinone Metabolite of Pentachlorophenol
    Chem. Res. Toxicol. 20, 913-919
  18. J.Gong, V.G.Vaidyanathan, X.Yu, T.W.Kensler, L.A.Peterson and S.J.Sturla (2007) Depurinating Acylfulvene-DNA Adducts: Characterizing Cellular Chemical Reactions of a Selective Antitumor Agent
    J. Am. Chem. Soc., 129, 2101-2111.
  19. D.Lawrence, V.G.Vaidyanathan and B.U.Nair (2006) Synthesis, characterization and DNA binding studies of two mixed ligand complexes of ruthenium(II)
    J. Inorg. Biochem. 100, 1244-1251 (Top 25 hottest articles in J Inorg. Biochem).
  20. V.G.Vaidyanathan, T.Weyhermuller, B.U.Nair and J.Subramanian (2005) DNA Damage Induced by a Chromium (III) Schiff Base Complex is Reversible under Physiological Condition
    J. Inorg. Biochem. 99, 2248-2255.  (Top 25 hottest articles in J Inorg Biochem).
  21. V.G.Vaidyanathan and B.U.Nair (2005) Synthesis, Characterization and Electrochemical Studies of Mixed Ligand Complexes of Ruthenium(II) with DNA
    Dalton Trans., 17, 2842-2848.
  22. V.G.Vaidyanathan and B.U.Nair (2005) Platinum(II) based Molecular Light Switch for Proteins.
    Eur. J. Inorg. Chem. 18, 3756-3759. 
  23. R.N.Patel, N.Singh, K.K.Shukla, J.Niclós-Gutiérrez, A.Castineiras, V.G. Vaidyanathan and B.U.Nair (2005) Synthesis, Characterization and biological activities of two copper(II) complexes with diethylenetriamine and 2,2′-bipyridine or 1,10-phenanthroline as ligands
    Spectrochim. Acta Part A: Mol. and Biomol. Spectroscopy, 62, 261-268 (Top 25 hottest articles in Spec. Chim. Acta).
  24. V.Uma, V.G.Vaidyanathan and B.U.Nair (2005) Synthesis, structure and metallonuclease activity of copper(II) complexes of terpyridine derivatives
    Bull. Chem. Soc. Jpn. 78, 845-850.
  25. V.G.Vaidyanathan and B.U.Nair (2004) Nucleobase Oxidation of DNA by Chromium(III) terpyridyl derivatives
    Eur. J. Inorg. Chem., 19, 1840-1846.
  26. V.G.Vaidyanathan and B.U.Nair (2003) Synthesis, Characterization and binding studies of Chromium(III) complex having an intercalating ligand with DNA
    J. Inorg. Biochem. 95, 334-342.
  27. V.G.Vaidyanathan and B.U.Nair (2003) Synthesis, Characterization and binding studies of Chromium(III) complex having tridentate ligand with DNA
    Eur. J. Inorg. Chem. 19, 3633- 3638.
  28. V.G.Vaidyanathan and B.U.Nair (2003) Photooxidation of DNA by Cobalt (II) tridentate complex
    J. Inorg. Biochem. 94, 121- 126.
  29. V.G.Vaidyanathan and B.U.Nair (2003) Oxidative Cleavage of DNA by Copper(II) tridentate complex
    J. Inorg. Biochem. 93, 271-276.
  30. V.G.Vaidyanathan and B.U.Nair (2002) Synthesis, Characterization and DNA binding studies of a ruthenium (II) complex
    J. Inorg. Biochem. 91, 405-412
  31. V.G.Vaidyanathan, R.Vijayalakshmi, V.Subramanian and B.U.Nair (2002) Synthesis, Characterization and Binding of [Cr(naphen)(H2O)2]+ with DNA: Experimental and Modeling Study
    Bull. Chem. Soc. Jpn. 75, 1143-1149
Honors and Awards
  • Recipient of Ramanujan Fellowship from Department of Science and Technology (DST), India (2011).
  • Recipient of "ACS Young Investigator Award-3rd Prize" from American Chemical Society (ACS) Division of Chemical Toxicology, Denver, Colorado, USA (2011)
  • Recipient of NSF “Experimental Program to Stimulate Competitive Research (EPSCoR)” Seed grant from Proteomics Division, Brown University (2009).
  • Travel Grant Award from CSIR to attend First Asian Meeting in Bioinorganic Chemistry, Okasaki, Japan (2003) (Participated as Graduate Student delegates from India).
  • Qualified as “Junior Research Fellow in National Eligibility Test (CSIR-NET)” conducted by Council of Scientific and Industrial Research (CSIR), Govt. of India (2000).
  • "Best Chemistry Student Award" in undergraduate Chemistry (1998)
PhD students

 

1. Nandhini T., Junior Research Fellow.,
MSc., Chemistry University of Madras (2014)
(Gold Medalist, D.G. Vaishnav College Chennai)
Project title: