CSIR-Central Leather Research Institute
Dr.M.K. Gowthaman
Sr. Principal Scientist
Academic Qualifications
    Ph.D
Contact Details
Dr.M.K. Gowthaman
Sr. Principal Scientist
Head
Biochemistry & Biotechnology, Biological Material Laboratory
CSIR-Central Leather Research Institute
Adyar, Chennai – 600 020
e-mail: mkgowthaman@clri.res.in, mkgowthaman@yahoo.com,
Phone:44-24437136


Education:

  • PhD  Chemical Engineering (1991) – Indian Institute of Technology (IIT), Madras, India.
  • M.Tech Chemical Engineering (1983) – A.C. College of Technology, Anna University, Chennai, India.
  • B.E. Chemical Engineering (1981) – Annamalai University, Annamalai Nagar, India.

Objective

Pursue teaching and research programs in Biotechnology to a high order of excellence with the experience gained in microbial enzyme technology, submerged, solid state fermentation and scale up of bioprocesses.

Career Summary

  • Twenty five years of experience in teaching courses in Chemical Engineering, Biotechnology, Food technology and Leather biotechnology.
  • Twenty five years of research experience in enzyme and fermentation technology; Supervised three PhD students and several students’ post graduate degrees.
  • Helped  to establish pilot scale fermentation facility at 500 L scale for upstream and downstream processing and currently coordinating it.
  • Six patents, five processes, 32 publications in international journals, book chapters and several conference presentations

Teaching and Research Interests:

  • Bioprocess Engineering & Upscaling
  • Chemical Engineering
  • Food Technology
  • Solid State Fermentation and Submerged Fermentation
  • Enzyme Technology

PhD students supervised

                    Degree awarded – 3                                        In progress - 2

Career Experience:

Central Leather Research Institute, Chennai – 600020, INDIA
(A constituent laboratory of Council of Scientific and Industrial Research, CSIR, Govt. Of India)
Principal Scientist, Senior Principal Scientist and Coordinator, Enzyme Upscaling Facility (July 2002-Date)
Responsibilities:

  • Nodal officer for New Millennium Indian Technology Leadership Initiatives (NMITLI) project “Biotechnology for cleaner leather processing” since 2010.
  • Coordinator for 500 L fermentation upscaling facility since 2008 to help develop scale up production of enzymes (proteases, lipases, amylases, tannase) for application in leather  and other industries.
  • Coordinator for the CSIR programme on Youth for Leadership in Science (CPYLS) during 2007-10, for state toppers in the X standard Examinations of CBSE, State, Matriculation, Anglo-Indian and ICSE Boards.
  • Chairman of Core Committee for organisation of CSIR Foundation Day 2011 celebrations.
  • Teaching Advanced Leather Biotechnology subject for B.Tech and M.Tech (Leather Technology) students.
  • Recognised PhD guide by Anna University and University of Madras.


University of Waterloo, Ontario, N2L3G1, Canada

Department of Chemical Engineering
Post Doctoral Research Associate on deputation (April 1999 – April 2000)
Responsibilities:

  • Research on heterologous lysozyme production from Aspergillus niger and  cellulase production from Neurospora sitophila by solid state fermentation.

Central Food Technological Research Institute (CFTRI), Mysore – 570020, India
(A constituent laboratory of Council of Scientific and Industrial Research, CSIR, Govt. Of India)
Senior Scientist and Principal Scientist (July 1993 – June 2002)
Responsibilities:

  • Co-investigator in DBT project “Computer control of fermentation process”.
  • Member of DBT project “Engineering aspects of solid state fermentation”.
  • Develop processes for production of enzymes glucoamylase, amylase, pectinase by solid state fermentation (SSF).
  • Coordinator of SSF pilot scale facility for upscaling above enzymes.
  • Develop processes for production of lactic acid, xantham gum, xylitol and grain alcohol by submerged fermentation.
  • In-charge of developing the above processes in 15 L, 50 L and 200 L fermenters.
  • Supervising students in final semester projects for B.Tech, M.Tech, M.Sc degrees in Food Technology and Biotechnology, University of Mysore.
  • Teaching Industrial microbiology for M.Sc (Food Technology) students.
  • Executing contract work for a few companies in enzyme technology and scale up.


Central Food Technological Research Institute (CFTRI), Mysore – 570020, India

Research Associate (Jan 1991 – June 1993)
Responsibilities:

  • Planning and conducting research experiments for generating design criteria under the project “Engineering aspects of Solid State Fermentation”.
  • Design and fabrication of laboratory bioreactors for solid state fermentation.


Vorion Chemicals and Distilleries Pvt Ltd., Chingleput, Tamilnadu, India.

Assistant Chemical Engineer (May 1984 – Dec 1984)
Responsibilities:

  • Design and construction of a pilot scale anaerobic digester system for production of biogas from molasses-based distillery effluent.

Publications:

  1. Gowthaman MK, SK Rakshit, K Krishnaiah and A Baradarajan (1991). Studies on RTD and continuous culture (SCP) in cylindrical and tapered reactors. Bioprocess and Biosystems Engineering Vol 7 (1-2), 41-46.
  2. KSMS. Raghava Rao, Gowthaman MK, NP Ghildyal and NG Karanth (1991). A mathematical model for solid state fermentation in tray bioreactors. Bioprocess and Biosystems Engineering Vol 8 (5-6), 255-262.
  3. Gowthaman, M.K.,  KSMS Raghava Rao, N.P. Ghildyal and N.G. Karanth (1993). Gas concentration and temperature gradients in a packed bed solid-state fermentor. Biotechnology Advances. Vol. 11,  pp 611-620.
  4. Gowthaman, M.K., N.P. Ghildyal, KSMS Raghava Rao and N.G. Karanth (1993). Interaction and transport resistances with biochemical reaction in packed bed solid state fermentors: The effect of gaseous concentration gradients. J. Chemical Technology and Biotechnology. Vol. 56, 233-239.
  5. Ghildyal, N.P., M.K. Gowthaman,  KSMS Raghava Rao and N.G. Karanth (1994). Interaction of transport resistances with biochemical reaction in packed-bed solid-state fermentors: Effect of temperature gradients. Enzyme and Microbial Technology. Vol. 16, 253-257.
  6. Gowthaman, M.K., KSMS Raghava Rao, N.P. Ghildyal and N.G. Karanth (1995). Estimation of Kla in solid state fermentation using a packed bed bioreactor. Process Biochemistry. Vol. 30, pp. 9-15.
  7. KSMS Raghava Rao, N.P. Rastogi, M.K. Gowthaman and N.G. Karanth (1995). Aqueous two phase extraction for downstream processing of enzymes/cells. Advances in Applied Microbiology 41: 98- 171.
  8. Tanuja, S., N.D. Srinivas, KSMS Raghava Rao and M.K. Gowthaman (1997). Aqueous two-phase extraction for downstream processing of amyloglucosidase. Process Biochemistry 32: 635-641.
  9. M.K. Gowthaman, M.S. Prasad and N.G. Karanth (1999). Fermentation (Industrial): Production of xanthan gum. Encyclopaedia of Food Microbiology, Ed. K. Robinson Richard, Elsevier, page 699.
  10. Veena Nadig, M.K. Gowthaman and NGK Karanth (2000). Scale up studies for the production of biosurfactant in a packed column bioreactor. Bioprocess Engineering, 22: 95 – 99.
  11. Sudha Krishnan, M.K. Gowthaman and NG Karanth (2001). Chitosan-treated polypropylene matrix as immobilization support for lactic acid production using Lactobacillus plantarum NCIM 2084. Journal of Chemical Technology and Biotechnology, 76: 461-468.
  12. M.K. Gowthaman, Chundakkadu Krishna and M. Moo-Young (2001). Fungal Solid State Fermentation – An Overview. In: Applied Mycology and Biotechnology, Ed. D.K. Arora and G.G. Khachatourians, Elsevier Science Vol.1, 305-359.
  13. M. Gyamerah, Gowthaman Merichetti*, O. Adedayo, J.M. Scharer and M. Moo-Young (2002). Bioprocessing strategies for improving hen egg-white lysozyme (HEWL) production by recombinant Aspergillus niger HEWL WT-13-16. Applied Microbiology and Biotechnology, 60: 403-407.
  14. K. Thirunavukarasu, N.G. Edwinoliver, S. Duraianbarasan, M.K. Gowthaman, H. Iefuji and N.R. Kamini (2008) Removal of triglyceride soil from fabrics by a novel lipase from Cryptococcus sp. S-2 Process Biochemistry 43: 701-706.
  15. N.G. Edwinoliver, K. Thirunavukarasu, S. Purushothaman, M.K. Gowthaman and N.R. Kamini (2009). Corn steep liquor as a nutrition adjunct for the production of Aspergillus niger lipase and hydrolysis of oils thereof. Journal of Agricultural and Food Chemistry, 57: 10658–10663.
  16. N.G. Edwinoliver, K. Thirunavukarasu, R.B. Naidu, M.K. Gowthaman, T. Nakajima Kambe and N.R. Kamini (2010). Scale up of a novel tri-substrate fermentation for enhanced production of Aspergillus niger for tallow hydrolysis. Bioresource Technology, 101: 6791-6796.
  17. Chandini S., Kumar , L. Jaganmohan, Rao , M.K., Gowthaman , D.J., Haware , R., Subramanian (2011). Enzymatic treatment to improve the quality of black tea extracts. Food Chemistry, 127:1039-1045
  18. Dinesh Prasad, Rishikesh K. Gupta,G.S. Venkataratnam,N.R. Kamini and M.K. Gowthaman (2011). Utilization of Bahera Fruits for Production of Tannase and Gallic Acid by Aspergillus heteromorphus MTCC 5466 and Synthesis of Propyl Gallate. Global Journal of Biotechnology & Biochemistry 6 (3): 119-128.
  19. Dinesh Prasad, Rishikesh K Gupta, N.R. Kamini and M.K. Gowthaman (2011). Microbial Tannases - An Overview. Monograph by  LAP LAMBERT Academic Publishing GmbH & Co. KG, Germany (ISBN 978-3-8465-1008-7).
  20. Dinesh Prasad, R.K. Gupta, G. Mathangi, N.R. Kamini and M.K. Gowthaman (2012). Advances in Production and Characteristics Features of Microbial Tannases: An Overview. Current Trends in Biotechnology and Pharmacy 6: 145-165.
  21. E. Nakkeeran, M.K. Gowthaman, S. Umesh-Kumar, R. Subramanian (2012). Techno-economic analysis of processes for Aspergillus carbonarius polygalacturonase production. Journal of Bioscience and Bioengineering 113 (5): 634-40.
  22. T. Nakajima-Kambe, N.G. Edwinoliver, H. Maeda, K. Thirunavukarasu, M.K. Gowthaman, K. Masaki, S. Mahalingam and N.R. Kamini (2012). Purification, cloning and expression of an Aspergillus niger lipase for degradation of poly(lactic acid) and poly(ε-caprolactone). Polymer Degradation and Stability 97: 139-144.
  23. M. Aarthy, P. Saravanan, M.K. Gowthaman,  N.R. Kamini (2014). Enzymatic transesterification for production of biodiesel using yeast lipases: An overview. Chemical Engineering Research and Design 92: 1591-1601.
  24. S. Pavithra, R. Ramesh, M. Aarthy, N. Ayyadurai, M.K. Gowthaman, N.R. Kamini (2014). Starchy substrates for production and characterization of Bacillus subtilis amylase and its efficacy in detergent and breadmaking formulations. Starch 66: 976-984.
  25.  P. Saravanan, T. Shiny Renitha, M.K. Gowthaman, N.R. Kamini (2014). Understanding the chemical free enzyme based cleaner unhairing process in leather manufacturing. J. Cleaner Production 79 (15):  258–264.
  26. R. Ramesh, M. Aarthy, M. K. Gowthaman, K. Gabrovska, T. Godjevargova  and N. R. Kamini(2014). Screening and production of a potent extracellular Arthrobacter creatinolyticus  urease for determination of heavy metal ions. Journal of Basic Microbiology 54: 285-295.
  27. Thirunavukarasu K, Purushothaman S, Gowthaman MK, Nakajima-Kambe T, Kamini NR. Utilization of fish meal and fish oil for production of Cryptococcus sp. MTCC 5455 lipase and hydrolysis of polyurethane thereof (2015). J Food Sci Technol. 52(9):5772-80. 
  28. T. Shiny Renitha, J. Sridevi, M. K. Gowthaman, N. R. Kamini, B. Ramanaiah and P. Saravanan (2015). Wealth from waste – beef extract for microbiological media from tannery solid waste. RSC Advances., 5, 9891.
  29. R. Ramesh, P. Puhazhselvan, J. Kumar, M.K. Gowthaman, S.F. D’Souza and N.R. Kamini (2015). Potentiometric biosensor for determination of urea in milk using immobilized Arthrobacter creatinolyticus urease. Material Science and Engineering C 49: 786-792.
  30. Ajitha Pandi, Sathya Ramalingam, Jonnalagadda Raghava Rao, Numbi Ramudu Kamini and Marichetti Kuppuswami Gowthaman (2016). Inexpensive α-amylase production and application for fiber splitting in leather processing. RSC Advances, 2016, 6, 33170 – 33176.
  31. Mayilvahanan Aarthy, Palanivel Saravanan, Niraikulam Ayyadurai , Marichetti Kuppuswami Gowthaman, Numbi Ramudu Kamini . A two step process for production of omega 3-polyunsaturated fatty acid concentrates from sardine oil using Cryptococcus sp. MTCC 5455 lipase. Journal of Molecular Catalysis B: Enzymatic 125 (2016) 25–33.
  32. G. Krishna Priya, Javid M. Mohammed Abu, A. George, M. Aarthy, Anbarsan S. Durai, N.R. Kamini, M.K. Gowthaman, R. Aravindhan, S. Ganesh, R. Chandrasekar, N. Ayyadurai (2016). Next generation greener leather dyeing process through recombinant green fluorescent protein. J. Cleaner Production (in press).

Selected conference papers:

  1. V.Prabahwathi & M.K.Gowthaman. "Novel thermostable protease from a bacterial strain for dehairing process". Innovations in leather science and technology held on 24th April 2006 at CLRI, Adyar, Chennai 600 020.
  2. V.Prabahwathi & M.K.Gowthaman. "Purification and characterization of a multifunctional extracellular protease from Bacillus pumilus".    3rd BRSI Convention and international conference on exploring horizons in Biotechnology: A global venture at Sardar Patel University, Vallabh Vidyanagar, Gujrat during November 2-4, 2006
  3. Dinesh Prasad, R.K. Gupta, R.B. Naidu, N.R. Kamini and M.K.Gowthaman “Solid state fermentation of tannase by a new fungal isolate”.  Association of Microbiologists of India (AMI) 48th Annual Conference, December 18 – 21, 2007, Indian Institute of Technology Madras, Chennai- 600 036.
  4. V.Prabhawathi & M.K.Gowthaman.  Partitioning of protease by aqueous two phase extraction.  International Conference on New Horizons in Biotechnology, November 26-29, 2007, Trivandrum.
  5. M.K. Gowthaman. Invited lecture on “Trends and Prospects in solid state fermentation” at the 48th Annual Conference of Association of Microbiologists of India (AMI), December 18– 21, 2007, Indian Institute of Technology Madras, Chennai- 600 036.
  6. A cost effective medium for protease production from a Bacillus isolate. Rishikesh Kumar Gupta, V. Prabhawathi, Dinesh Prasad, R.B. Naidu, N.R. Kamini and M.K. Gowthaman. AMI conference held at IIT, Madras in Dec 2007.
  7. S.Sivasubramanian, B.Indrakumar, T.Napolean Bonaparte, P.Prakash, M.Padmalosini, S.Muthukumar, N.R.Kamini, M.K.Gowthaman, R.Puvanakrishnan (2007). Scale up of a bacterial protease and its downstream processing. International conference held in Kalasalingam University, Srivilliputhur, 2007.
  8. N. G. Edwin Oliver, K. Thirunavukarasu, Usha Ramamurthy, A. Rajaram, R.B. Naidu, M.K. Gowthaman, and N.R. Kamini. “A novel trisubstrate fermentation for lipase production from Aspergillus niger for its application in degreasing”. Poster presented in LERIG 2008 held on January 27-30, 2008 at CLRI.
  9.  N. Kayalvizhi, T. Anthony, S. Sathyavathi, R. Preeti,  M.K. Gowthaman, P. Saravanan, N.K.Chandrababu, T. Ramasami, A. Amudeswari and P. Gunasekaran. Biodegradation and Biopreservation of animal skin.44th Leather Research Industry get-together (LERIG 2010) BIOTAN “Bio-related Innovative options of Tannery applications” CLRI Chennai. 
  10.  Ajitha Pandi, Keerthana S, Naidu R.B., Kamini, N.R. and Gowthaman M.K (2016). Fungal production of laccase by two strategies - A comparison. International Conference on Recent Trends in Biosciences, April 7-9, 2016, Alagappa University, Karaikudi, Tamilnadu, India.

Patents

  1. Arulmuthu ER, Marichetti KG, Nandan PG, Mahesh CM and Ganesh KN. Issue date: 17th May 2005. US patent 6,893,849, Fermentation process for the production of xylitol from Pichia sp.”
  2. Eugene Raj A, Venkatesh KS, Ravi Kumar K, Umesh Kumar S, Misra MC, Ghildyal NP, Karanth NG, Gowthaman MK “An improved process for the production of pectinase enzyme from Aspergillis carbonarius”, NF 96/2002.
  3. S. Sivasubramanian, R.B. Naidu, N.R. Kamini, M.K. Gowthaman, P. Saravanan, S. Ramalingam, R. Ramesh, J. Kanagaraj, N.K. Chandrababu, Y.K. Saikumari, P. Balaram and R. Puvanakrishnan (2007). A novel alkaline protease and a process for the preparation thereof. Indian Patent Application No. 2375/DEL/2007.
  4. Dinesh Prasad, Rishikesh Kumar Gupta, Veluchamy Prabhawathi, Nesamony Gnanadhas Edwin Oliver, Ramachandra Boopathy Naidu, Numbi Ramudu Kamini,  Narasimhan Kannan Chandrababu, Palanivel Saravanan, Marichetti Kuppuswamy Gowthaman (2009). A Novel pH And Thermostable Tannase From A Fungal Source And A Process For The Production Thereof. Indian Patent 2372DEL2009
  5. Veluchamy Prabhawathi, Gupta Rishikesh Kumar, Prasad Dinesh, Sambamurthy Durai Anbarasan, Sundaramoorthy Sundarapandiyan, Nesamony Gnanadhas  Edwin Oliver, Naidu  Ramachandra Boopathy, Numbi Ramudu Kamini, Palanivel Saravanan, Marichetti Kuppuswamy Gowthaman (2011). A novel alkaline protease for industrial applications and a process for the production thereof. Indian Patent Application 0053NF2011.
  6. Nagarajan Kayalvizhi, Paramasamy Gunasekaran, Marichetti Kuppuswamy Gowthaman, Palanivel Saravanan, Ramakrishnan Ramesh, Narasimhan Kannan Chandrababu, Thirumalachari Ramasami, Anbazhagan Amudeswari, Asit Baran Mandal (2010). A novel bacteriocin and a process for the production thereof . Indian Patent 0781DEL2010/IN(31/03/2010).
  7. N.G. Edwinoliver, K. Thirunavukarasu, S. Purushothaman,  M.K. Gowthaman and N.R. Kamini. A process for the production of bacterial protease enzyme for industrial application. Indian patent 1181/DEL/2013.