A novel alkaline protease and a process for the preparation thereof
A novel alkaline protease exhibiting a feature of halotolerance and activity as well as stability in the presence of oxidants as well as surfactants is provided. While the protease exhibits elastolytic activity, there is no collagenolytic activity. The alkaline protease is prepared from a strain of Bacillus subtilis, designated asMTCC 5333, using submerged fermentation technique, wherein the production medium is prepared without using any common salt or alkali and the pH of the said medium is the neutral range. The protease finds potential application in soaking as well as dehairing operations in leather processing industry. It may also be used as an additive in detergent industry.
The invention has been applied for patent protection (Indian Patent application no. 2375Del2007; Patent pending).
A process for the production of an alkaline protease
The process relates to the production of an extracellular alkaline thermo tolerant protease from a bacterial strain of genus Alcaligens (CLRI BL11). The process enables a much higher yield (800-1200 Anson units per ml of culture filtrate), compared to those in the hithertoknown processes. This protease exhibits stability at a pH in the range of 7-13 and a temperature of 50-65oC. The enzyme finds enormous application as a soaking and unhairing agent in leather processing industry. It also finds potential application in dairy industry for making cheese. Moreover, it may be used to prepare protein hydrolysates by controlling hydrolysis of proteins present in a substrate.
The invention has been applied for patent protection.(Indian Patent application no. 1122Del1999, Patent pending).
A process for the preparation of a proteolytic enzyme from a novel strain of fungus Paecilomyces lilacinus 
The process relates to the production of a proteolytic enzyme from a fungal strain Paecilomyces lilacinus (MTCC 2835) using solid state fermentation technique. The enzyme exhibits activity at a pH in the range of 6-13 and temperature in the range of 25-70oC. The shelf life of the enzyme, unlike those of the conventional proteases, is as long as 2 years, when maintained at 5-20oC. The enzyme finds enormous application as bate, soaking agent as well as depilant in leather processing industry. It may also be used to treat leather industry byproducts such as fleshings, shavings etc to make value added products. Further, the enzyme may be used in meat industry, dairy, distillery, jute industry, food and fruit processing industry. It may also be used as an additive for enhancing efficacy of detergents.
The invention has been applied for patent protection.(Indian Patent application no. 377Del2000, Patent pending).
A process for the production of a novel lipase
The process relates to the production of lipase from a bacterial strain Ralstonia pickettii (CLRI BTL1). Unlike the conventional lipase, this enzyme exhibits stability over a wider range of pH (2-10) and temperature (4-80oC). The shelf life of the enzyme is around 90 days. The enzyme finds enormous application as a degreasing agent in leather processing industry. It may also be used as an additive to laundry detergent formulations. It has been found to be able to remove olive oil from cotton fabrics with better efficiency in the presence of available detergents. Moreover, it is a potential hydrolyzing agent for industrially important fats and oils, while producing fatty acids. It may find use in esterification, transesterification and interesterification reactions. It has also potential use as a digestive aid in pharmaceutical industry.
The invention has been applied for patent protection.(Indian Patent application no. 1555Del1999, Patent pending).
An improved process for the production of stable lipase
The process relates to the production of lipase from a fungal strain of Aspergillus niger (CLRI BTFL). Unlike the conventional lipase, this enzyme exhibits stability over a wider range of pH (2-11) and temperature (4-60oC). The enzyme finds enormous application as a degreasing agent in leather processing industry. It may also be used as an additive to laundry detergent formulations. It may also be used for the production of industrially important fatty acids from fats and oils in oleochemical industry. Chemical industries can use this enzyme for synthesis of esters and acylation of glucose. Moreover, it finds application in food and pharmaceutical industry for interesterification of butterfat, resolution of racemic mixtures and synthesis of peptides. It may even be used in the generation of dairy flavours for dairy industry.
The invention has been applied for patent protection.(Indian Patent application no. 1556Del1999, Patent pending).
A novel protease for industrial applications
The invention relates to an extracellular enzyme protease obtained by growing the culture of Pseudomonas aeruginosa isolated from vermiculture pit soil and deposited in MTCC, IMTECH, Chandigarh with designation MTCC 5270, in production medium of pH 7.0 and containing soybean meal and tryptone as raw materials, at 30°C for 72 h. Different agricultural products/ byproducts are used as protein sources. The main advantage of the invention is the use of simple substrates like soya flour, bengal gram flour and wheat bran for the production of protease.
The partially purified non-collagenolytic, calcium independent protease with molecular weight 60 kDa has activity in pH range of 6.0 – 11.0 and temperature range of 25-650C; stability in pH range of 6.0-10.0 and temperature 25-45°C. The protease activity is retained for 8 months when stored at ambient temperature.
The protease of the present invention finds enormous potential application in leather processing industry as an eco-benign option for dehairing of hides and skins with enzyme alone without supplementation of any chemical like lime or sodium sulphide. The use of protease reduces the dehairing time and makes dehairing more efficient resulting in better grain quality of leather. It may also be used in soaking process of leather manufacturing. The application of the enzyme avoids the pollution produced during pretanning operation associated with conventional method, thus making the enzyme, an ecofriendly product.
Further, the enzyme finds application as an additive in detergent and also for recovery of silver from waste X-ray and photographic films. It may also be used for preparations of protein hydrolysates. Yet another potential application of the protease of the present invention is in pharmaceutical industries and food processing industry.
The invention has been applied for Patent protection (Indian Patent application no. 2471DEL06).
A novel alkaline protease and a process for the production thereof
A novel alkaline protease has been produced from a Aspergillus source (MTCC5152). It is essentially an extra cellular alkaline protease active in a wide range of ph and temperature conditions. The invention further provides a process for the preparation of the novel protease using solid state fermentation or liquid fermentation. The extra cellular alkaline protease produced by the process of the present invention has potential
Application in leather industry as a soaking and unhairing agent in the beamhouse processing. It may also be used for the purpose of food processing. The protease may also be used to prepare protein hydrolysates by controlling the hydrolysis of proteins present in a substrate. Being an alkaline protease, this enzyme may even be used as a supplement in laundry detergents.
The invention has been applied for Patent protection (Indian Patent application no. 0391DEL2007). |
