A process for the preparation of reconstituted collagen sponge
A process for the preparation of collagen-chitosan bilayer material
A process for extraction of atelopeptide collagen from a collagenous source by microbial treatment
An improved process for the preparation of fibroplast 
The fibroplast is non-immunogenic, semitransparent cover, which seals the wound while breathing like skin, thereby healing without scar formation. Collagenous substance, extracted from a collagenous source, is subjected to extensive purification to remove non-collagenous matters and the pure soluble collagen is cast into fibroplasts of desired size and shape. The resulting reconstituted fibroplast is subjected to crosslinking and sterilization. The product finds enormous application as a wound dressing aid for open wounds such as second degree burn, open raw surface, ulcers and the like. It enhances healing of wound, burn, excision wounds etc. Moreover, it prevents excessive evaporation of fluid and controls the infection. A unique feature of the product is that it exhibits better biocompatibility because of the purity of collagen. Further, the wound covered with a fibroplast can be inspected and monitored easily without removing it, because of the excellent transparency of the sheet.
An Indian patent has been granted (Patent no. IN192397) for this invention.
The technology has already been transferred and the product is also available in Indian market.
A process for the preparation of reconstituted collagen sponge
Pure collagenous substance is treated with surfactants in the presence of crosslinkers and pharmaceutically as well as pharmacologically active components under controlled conditions. The resulting mixture is lyophilized and sterilized. The surfactant enables the product to possess large pore structure capable of entrapping different drugs and controlled release of entrapped drugs is ensured. The product has potential application as wound dressing aid, bone-cartilage substitute, surgical tampons, laparatomy pads anda container for drug release. It serves as a better scaffolding for re-epithelization or good granulation of tissue to ensure faster recovery of wound as well as relief from pain. It prevents excess evaporation of fluid from wound ensuring minimum fluid loss from the wound site and also prevents any infection, thereby enhancing healing of wounds, burns, excision wounds.
The invention has been applied for patent protection.(Indian Patent application no. 369Del2000, Patent pending).
An improved process for the preparation of collagen sponge
A process for the preparation of collagen sponge without lyophilisation is disclosed, whereby the collagen solution obtained from a conventional collagenous source is treated with a non-ionic wetting agent under specified conditions to enable the treated solution to be air dried, thereby reducing the high cost involved in conventional lyophilisation. It is envisaged to have enormous potential application in the pharmaceutical industry in preparing cost effective collagenous biomaterial useful for medical applications. The sponge obtained by this process can be used for different medical applications including burn management, wound cover, drug delivery device etc.
The invention has been applied for Patent protection (Indian Patent application no. 461DEL2003;Patent pending)
A process for the preparation of plant based reconstituted collagen substratum
The process involves purification of proteolytic enzyme solubilized collagen and preparation of Aloe vera extract from its parenchymal tissue. The purified collagen and Aloe vera extract are mixed together and reconstituted. Subsequently, the solution mixture is spread to form sheets, followed by sterilization to obtain plant based reconstituted collagen substratum. The product finds potential medical application as biological cover for non-healing ulcers as well as burn injuries.
The invention has been applied for Patent protection (Indian Patent application no. 84DEL2002; Patent pending).
A process for the preparation of reconstituted collagen substratum
The invention relates to the preparation of reconstituted collagen substratum from a human source. The process essentially involves isolation of Collagen Type IV, which is layered on the substratum made up of Type I & III collagen, thereby facilitating an efficient growth of keratinocytes. The product finds potential medical application as autograft for burn injuries of any degree. This may also be used as an effective permanent dressing material for wounds and burns. The major advantage of this product is that since it is prepared from human compatible source, no adverse/allergic reaction is envisaged at the human site of use and healing is expedited.
The invention has been applied for Patent protection (Indian Patent application no. 709DEL2000; Patent pending).
A process for the preparation of biopolymer scaffold for medical applications.
The invention relates to a scaffold to aid in the tissue regeneration of tissue, such as bone, cartilage or soft tissue and as a container for delivery of bioactive molecules like growth factors. The scaffold is formed by the combination of the soluble biopolymer with a glycol, alkaline earth metal chloride and a cross linker. It comprises a polymer network having active agents, such as wound healing agents, incorporated therein. The compositions are suitable for use as or in wound dressings as it comprises methods and compositions for treating wounds. The matrix may be formed into any desired shape for treatment of wounds. The composition comprises a single density foam, either with or without cell seeding, which is not freeze dried, can be used to build living tissue equivalents or model tissue systems.
Indian Patent has been granted for the invention (Indian Patent No. 199822).
A process for the preparation of a novel collagen scaffold useful for wound dressing.
The process involves purification of collagen from fetal calfskin by conventional method. Development of antibacterial agent loaded alginate microsphere by water/oil emulsion technique through ionic gelation of alginate, thus entrapping antibacterial agent in the alginate polymer matrix followed by rigidization and drying of the drug loaded microspheres. The prepared drug loaded microsphere was impregnated in the prepared collagen and subsequently initiating fibrillation. The drug impregnated fibrillated collagen was cast as a thin scaffold under controlled temperature and aseptic environment. The developed novel collagen scaffold impregnated with antibacterial agent delivers the drug in a controlled manner thus reducing frequency of dressing and makes wound assessment easier.
The invention has been applied for Patent protection (Indian Patent application no. 314DEL2004;Patent pending).
A process for the preparation of collagen-chitosan bilayer material.
Collagen purified to the necessary extent (with or without telopeptide) in acid soluble form at a concentration of 1-10 mg/ml is made to gel around physiological conditions and crosslinked with appropriate chemicals in very low concentrations of 10-5 to 10-2 percent. An attachment to a layer of chitosan is effected at the same time resulting in a bilayer material. The composite matrix is further processed as appropriate to make four types of bilayer matrices ie. collagen film – chitosan film, collagen sponge – chitosan film, collagen film – chitosan sponge and collagen sponge – chitosan sponge. The bilayer material is envisaged to have enormous application in pharmaceutical industry as wound healing agent. It also finds potential application in biomedical area as efficient tissue engineering matrix.
The invention has been applied for Patent protection (Indian Patent application no. 321DEL2004;Patent pending).
A process for extraction of atelopeptide collagen from a collagenous source by microbial treatment.
The invention relates to an extraction process to derive collagen fibres of high tensile strength from collagenous tissue. This microbial extraction procedure ensures degradation of non-collagenous components, leaving the collagenous matter intact. The resulting atelopeptide collagen exhibits largely monomeric triple helical conformation.The collagen is rendered non-immunogenic by the removal of certain terminal peptide chains. The non-cytotoxic fibres can be fabricated into various physical forms for biomedical applications.
It is envisaged to have enormous potential application in the pharmaceutical industry for preparing cost effective collagenous biomaterials useful for medical applications. Moreover, the atelopeptide collagen extracted by this process can be used as a suture material for surgical applications. It may also be molded in diverse shapes, ensuring its use as an ideal carrier, wound cover or drug delivery scaffold, physically cut fibres with any sharp instrument including a sterile scalpel to suit the dimensions of a punctual plug for medical applications.
Ophthalmic related
A process for the preparation of ionically charged collagen sponge
A process for the preparation of viscoelastic, bio-erodible ophthalmic shield
A process for the preparation of bio-erodible insert for ophthalmic applications
A process for the preparation of ionically charged collagen sponge
The process relates to modifying the polar sites of collagen using charged ligands under inert atmosphere to prepare ionically charged collagenous solution, which is cast into sponge of desired shape and size after incorporating foaming agent, crosslinkers and pharmaceutically active components. The resulting collagen sponge is either cationic or anionic at physiological pH and it can be effectively used at the sites of ionic nature. The ionic property of the sponge is of paramount importance in several surgical requirements where charge plays an important role. The ionically charged collagen sponge finds potential application as corneal shields in ophthamological treatment. It may also be used as effective haemostat for wound dressing. It may even be used as bone-cartilage substitute, surgical tampons, laparatomy pads and containers for drug release. The product is capable of entrapping different drugs and the controlled release of entrapped drugs is facilitated. Moreover, the soluble collagen, which is obtained as an intermediate product while making the sponge, finds a very high demand as a component of skin care products such as creams, shampoo etc, in the cosmetic and pharmaceutical industry.
An Indian patent has been granted (Patent no. IN191590) for this invention.
A process for the preparation of viscoelastic, bio-erodible ophthalmic shield.
A process for the preparation of visco-elastic bio-erodible ophthalmic shield is disclosed, whereby pure collagen is obtained from bovine tissues by pH alteration and enzyme treatment this is followed by succinylation with succinic anhydride. The pure succinylated collagen is treated with hydrophilic polymers and antioxidant to get a viscoelastic solution. The solution is dried in moulds in a dust free chamber, cross-linked with UV irradiation and sterilised to get a bio-erodible ophthalmic shield for the treatment of infected eyes, injured eyes, dry eyes and traumatic and non-traumatic eyes. This can include one or more therapeutically active substances that will be released in the eye. The ophthalmic shield is biocompatible, bio-erodible, visco-elastic and transparent which can be used for ophthalmic application.
The invention has been applied for Patent protection (Indian Patent application no. 313DEL2004).
A novel bio-erodible insert for ophthalmic applications and a process for the preparation thereof
A novel bio-erodible insert for ophthalmic applications has been developed. The invention further relates to a process for the preparation of the insert. In the said process, collagen is treated with organic polar solvents, hydrophilic polymers and therapeutically active substances under controlled conditions. The resulting solution is air dried in a dust free chamber to make collagen film. This film is cut into shape to obtain insert, which is subjected to cross-linking with UV irradiation followed by conventional sterilization. The resulting insert is very effective for temporary punctual occlusion in various corneal conditions and is very effective for treating dry eye syndrome resulting due to occupational conditions.
The invention has been applied for Patent protection (Indian Patent application no. 2064DEL2006).A process for the preparation of transparent soft collagen film
A process is provided for the preparation of anionically charged transparent as well as soft collagenous substrate for external application in the medical treatment for eye disease. The process is envisaged to have enormous application in the pharmaceutical industry for preparing ophthalmic substances in the form of films, which can be applied topically to the cornea or instilled in the space between eyeball and lower eyelid for curing eye disease. The ocular region covered with the film can be constantly inspected without disturbing the film as it is very thin and transparent.